Products
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Human Transforming Growth Factor β3(TGFB3) Protein, Recombinant
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P01T0005 |
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Other Names
ARVD; ARVD1; LDS5; RNHF; TGF-beta3
Background
The mature monomer of TGF-β3 is composed of 112 amino acids, and the monomer part is at the carboxyl terminal of the precursor protein of TGF-β3.The structure of TGF-β3 in human body is a 25ku dimer, which is crosslinked by disulfide bonds between two monomers with the same structure and molecular weight of 12.5ku.TGF-β3 signaling is mediated by membrane-bound serine/threonine kinase receptors that regulate the transcription of target genes through the Smads protein pathway, thereby mediating a series of TGF-β3 biological responses.
The protein is involved in embryogenesis and cell differentiation, and may play a role in wound healing. Studies have shown that the TGF-β family is the most closely known cytokine associated with scar formation. The TGF-β family is a feedback inhibition system. Although the amino acid homology of TGF-β1, TGF-β2 and TGF-β3 among each other is up to 70% , they play different roles in different cell types. TGF-β1 and TGF-β2 can promote collagen formation. while TGF-β3 can antagonize TGF-β1 and TGF-β2, suggesting that TGF-β1 and TGF-β2 are related to scar hyperplasia and TGF-β3 plays a role in scar regression.
Recent studies have shown that TGF-β 3, a member of the transforming growth factor family, can effectively promote the transformation process of adipose stem cells into chondrocytes, thereby promoting chondrogenesis.
Product Name
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Recombinant TGFB3 Protein
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Species
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Human
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Product Size
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10ug/50ug/100ug/1mg
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Protein sequence
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MKMHLQRALVVLALLNFATVSLSLSTCTTLDFGHIKKKRVEAIR
GQILSKLRLTSPPEPTVMTHVPYQVLALYNSTRELLEEMHGEREEGCTQENTESEYYA
KEIHKFDMIQGLAEHNELAVCPKGITSKVFRFNVSSVEKNRTNLFRAEFRVLRVPNPS
SKRNEQRIELFQILRPDEHIAKQRYIGGKNLPTRGTAEWLSFDVTDTVREWLLRRESN
LGLEISIHCPCHTFQPNGDILENIHEVMEIKFKGVDNEDDHGRGDLGRLKKQKDHHNP
HLILMMIPPHRLDNPGQGGQRKKRALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVH
EPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYV
GRTPKVEQLSNMVVKSCKCS
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Express region
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1-412aa
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Fusion Tag
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6x His-SUMO (N-terminus)
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Molecular Weight
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About 43.7 kDa |
Purity
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≥95% SDS-PAGE
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Physical property
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liquid or lyophilized powder
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Reconstitution
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Storage solution: We recommend using PBS or a suitable solvent according to the experimental requirements to prepare 1mg/mL storage solution, aliquot and store at -20 °C.
Working solution: According to the experimental requirement, dilute Storage solution. The working solution can be stored at 4°C for 2-3 weeks after dilution.
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Storage & stability
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The shelf life of liquid form is 6 months stored at -20 °C /-80 °C.
The shelf life of lyophilized form is 12 months stored at -20 °C /-80 °C.
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Result
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Reference
1. Debao Li,Xiaofei Ma,Tianlan Zhao. Mechanism of TGF-β 3 promoting chondrogenesis in human fat stem cells. Biochem Biophys Res Commun. 2020;530(4):725-731
2. 宋江南,李校堃,苏志坚,吴志玲,黄亚东.TGF-β3生物学特性、功能与临床应用前景.药物生物技术,2006(02):154-158.
3. 黄亚东,苏志坚,郑青,赵文,许华,吴晓萍,胡晓辉,李校堃,刘丙萍.人转化生长因子TGF-β_3基因合成及其在大肠杆菌中的表达.中国药科大学学报,2003(03):70-73.
4. Hakvoort T,Altun V,van Zuijlen P P,de Boer W I,van Schadewij W A,van der Kwast T H. Transforming growth factor-beta(1), -beta(2), -beta(3), basic fibroblast growth factor and vascular endothelial growth factor expression in keratinocytes of burn scars. European cytokine network,2000,11(2): 233-239.
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