E01A0007 Human Epinephrine/Adrenaline ELISA kit
Human Epinephrine/Adrenaline ELISA kit is suitable for the detection of samples from human species. Epinephrine/Adrenaline can also be called ADR, EPI.
Specifications of Human Epinephrine/Adrenaline ELISA kit
Product Information | |
Cat. No. | E01A0007 |
Product Name | Human Epinephrine/Adrenaline ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 25 ng/mL | 1 vial |
STANDARD C (0.5mL) | 50 ng/mL | 1 vial |
STANDARD D (0.5mL) | 100 ng/mL | 1 vial |
STANDARD E (0.5mL) | 250 ng/mL | 1 vial |
STANDARD F (0.5mL) | 500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
EPI ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EPI antibody and an EPI-HRP conjugate. The assay sample and buffer are incubated together with EPI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPI concentration since EPI from samples and EPI-HRP conjugate compete for the anti-EPI antibody binding site. Since the number of sites is limited, as more sites are occupied by EPI from the sample, fewer sites are left to bind EPI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPI concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Epinephrine/Adrenaline ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-108% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-102 | |
1:4 | 90-105 | |
1:8 | 89-109 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between EPI and analogues was observed. | |
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Summary of the Assay Procedure for Human Epinephrine/Adrenaline ELISA kit
Citations of Human Epinephrine/Adrenaline ELISA kit
E01A0007 has been referenced in the below publications:
The influences of stellate ganglion block on the level of stress hormone for patients of cephalic and facial postherpetic neuralgia.
Effects of hemodialysis on the cardiovascular function in patients with intradialytic hypertension and the related mechanism.
Changes of ET-1 and NO in the maintenance hemodialysis complicated with intradialytic hypertension.
