E01A0023 Human Anti Livin ELISA kit
Human Anti livin antibody ELISA kit is suitable for the detection of samples from human species. Anti livin antibody can also be called as Anti BIRC7; Anti KIAP; Anti LIVIN; Anti ML-IAP; Anti MLIAP; Anti RNF50; Anti baculoviral IAP repeat containing 7.
Product Information | |
Cat. No. | E01A0023 |
Product Name | Human Anti livin antibody ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Livin-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a Livin antigen and a Livin-Ab-HRP conjugate. The assay sample and buffer are incubated together with Livin-Ab-HRP conjugate in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for the HRP enzyme. The product of the enzyme-substrate reaction forms a blue-colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of the color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Livin-Ab concentration since Livin-Ab from samples and Livin-Ab-HRP conjugate compete for the Livin antigen binding site. Since the number of sites is limited, as more sites are occupied by Livin-Ab from the sample, fewer sites are left to bind Livin-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Livin-Ab concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-113% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-105 | |
1:4 | 92-107 | |
1:8 | 87-112 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Anti livin and analogues was observed. | |
E01A0023 has been referenced in the below publications：
Chronic atrophic gastritis Precancerous lesion of gastric cancer turbidity toxin intrinsic card with Livin Smac/DIABLO and Caspase-3 correlation studies.
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