BlueGene E01A0039 Human Total β Amyloid Protein ELISA Kit, Aβ ELISA

BlueGene Biotech Human Total β Amyloid Protein ELISA kit

E01A0039 Human Total β Amyloid Protein ELISA kit

Human Total β Amyloid Protein ELISA Kit is suitable for the detection of samples from human species. Total β Amyloid Protein can also be called Aβ, Aβ protein, Amyloid beta-peptide, Beta-amyloid peptide, Beta-amyloid protein, beta Amyloid Protein.

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Specifications of Human Total β Amyloid Protein ELISA kit

Product Information
Cat. No.	E01A0039
Product Name	Human Alkaline Phosphatase ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	5.0-100ng/mL
Sensitivity	1.0 ng/mL
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	5 ng/mL	1 vial
STANDARD C (0.5mL)	10 ng/mL	1 vial
STANDARD D (0.5mL)	25 ng/mL	1 vial
STANDARD E (0.5mL)	50 ng/mL	1 vial
STANDARD F (0.5mL)	100 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

AΒ ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AΒ. Standards or samples are then added to the microtiter plate wells and AΒ if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AΒ present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AΒ are added to each well to “sandwich” the AΒ immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AΒ and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AΒ concentration in each sample is interpolated from this standard curve.a

Quality Control on Human Total β Amyloid Protein ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-105%
Linearity	Diluent Ratio	Range %
	1:2	92-102
	1:4	91-107
	1:8	91-105
Specificity/Cross-reactivity	No significant cross-reactivity or interference between AΒ and analogues was observed.

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Summary of the Assay Procedure for Human Total β Amyloid Protein ELISA kit

Citations of Human Total β Amyloid Protein ELISA kit

E01A0039 has been referenced in the below publications:

The study of the Characteristics of Cognitive Impairment after Acute Mild-to-Moderate Ischemic Stroke and the Role of SAA and Aβ in Cognitive Impairment.