BlueGene: Bovine Total Adiponectin ELISA Kit, ADPN/AdipoQ/Acrp30 Assay Kit

BlueGene Biotech Bovine Total Adiponectin ELISA kit

E11A0125 Bovine Total Adiponectin ELISA kit

Bovine Total Adiponectin ELISA kit is suitable for the detection of samples from Bovine species. Total Adiponectin can also be called as 30 kDa adipocyte complement related protein; 30 kDa adipocyte complement-related protein; ACDC; ACRP 30; ACRP30; ADIPO; Adipocyte; Adipocyte C1q and collagen domain containing protein; Adipocyte complement related 30 kDa protein; Adipocyte complement related protein of 30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP 30, ACRP30, ADIPO, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, Adiponectin, AdipoQ, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPQTL1, ADPN, APM 1, apM-1, ApM1, Gelatin-binding protein.

Products

Specifications of Bovine Total Adiponectin ELISA kit

Product Information
Cat. NO.	E11A0125
Product Name	Bovine Total Adiponectin ELISA kit
Species	Bovine
Product Size	48 Tests / 96 Tests
Concentration	1-25 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

ADPN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADPN antibody and an ADPN-HRP conjugate. The assay sample and buffer are incubated together with ADPN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ADPN antibody binding site between ADPN from samples and ADPN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ADPN. As more sites are taken up by ADPN from the sample, fewer sites are left that can bind ADPN-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. This standard curve is interpolated to determine the ADPN concentration in each sample.

Quality Control on Bovine Total Adiponectin ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-106%
Linearity	Diluent Ratio	Range %
	1:2	93-103
	1:4	89-108
	1:8	86-109
Specificity/Cross-reactivity	No significant cross-reactivity or interference between ADPN and analogues was observed.