Horse Insulin Like Growth Factor Binding Protein 1 ELISA kit

BlueGene Biotech Horse Insulin Like Growth Factor Binding Protein 1 ELISA kit

E10I0062 Horse Insulin Like Growth Factor Binding Protein 1 ELISA kit

Horse Insulin Like Growth Factor Binding Protein 1 ELISA kit is suitable for the detection of samples from Horse species. Horse Insulin Like Growth Factor Binding Protein 1 ELISA kit can also be called IGFBP1, IBP-1, placental protein 12, PP12, IBP-1, IGF-binding protein 1, IGFBP-1.

Products

Specifications of Horse Insulin Like Growth Factor Binding Protein 1 ELISA kit

Product Information
Cat. No.	E10I0062
Product Name	Horse Insulin Like Growth Factor Binding Protein 1 ELISA kit
Species	Horse
Product Size	48 Tests / 96 Tests
Concentration	1.0-25 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IGFBP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IGFBP1 antibody and an IGFBP1-HRP conjugate. The assay sample and buffer are incubated together with IGFBP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGFBP1 concentration since IGFBP1 from samples and IGFBP1-HRP conjugate compete for the anti-IGFBP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGFBP1 from the sample, fewer sites are left to bind IGFBP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP1 concentration in each sample is interpolated from this standard curve.

Quality Control On Horse Insulin Like Growth Factor Binding Protein 1 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	86-109.73%
Linearity	Diluent Ratio	Range %
	1:2	91-109
	1:4	89-112
	1:8	87-115
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IGFBP1 and analogues was observed.