E01A0530 Human High molecular weight Adiponectin ELISA kit
The Human High molecular weight Adiponectin ELISA kit can be used to identify samples from the human species. High molecular weight Adiponectin can also be called HMW ADIPOQ, HMW ACDC, HMW ACRP30, HMW ADIPQTL1, HMW ADPN, HMW APM-1, HMW APM1, HMW GBP28, HMW adiponectin.
Product Information | |
Cat. No. | E01A0530 |
Product Name | Human High molecular weight Adiponectin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 µg/ml |
Sensitivity | 0.1 µg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 µg/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 µg/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 µg/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 µg/mL | 1 vial |
STANDARD E (0.5mL) | 10 µg/mL | 1 vial |
STANDARD F (0.5mL) | 25 µg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HMW ADNP ELISA kit uses an anti-HMW ADNP antibody and an HMW ADNP-HRP conjugate in a competitive enzyme immunoassay method. HMW ADNP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HMW ADNP antibody binding site between HMW ADNP from samples and HMW ADNP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HMW ADNP. Since the number of sites is limited, as more sites are occupied by HMW ADNP from the sample, fewer sites are left to bind HMW ADNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMW ADNP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HMW ADNP and analogues was observed. | |
E01A0530 has been referenced in the below publications：
Changes of microalbuminuria and serum high molecular weight—adiponectin in hypertensive patients with metabolic syndrome.
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