E01T0025 Human Thromboxanes B2 ELISA kit
The Human Thromboxanes B2 ELISA kit can be used to identify samples from the human species. Thromboxanes B2 can also be called TXB2, hromboxane-b, Thromboxane B2.
Specifications of Human Thromboxanes B2 ELISA kit
Product Information | |
Cat. No. | E01T0025 |
Product Name | Human Thromboxanes B2 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TXB2 ELISA kit uses an anti-TXB2 antibody and an TXB2-HRP conjugate in a competitive enzyme immunoassay method. TXB2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TXB2 antibody binding site between TXB2 from samples and TXB2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TXB2. Since the number of sites is limited, as more sites are occupied by TXB2 from the sample, fewer sites are left to bind TXB2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TXB2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Thromboxanes B2 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TXB2 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Thromboxanes B2 ELISA kit
Citations of Human Thromboxanes B2 ELISA kit
E01T0025 has been referenced in the below publications：
The Clinical Study on the effect of Kun Fu Kang Capsule on Red Cell Immunology with Chronic Pelvic Inflammatory Disease.
Correlativity between PGs(6-keto-PGF1α)levels and postpartum hemorrhage caused by uterine inertia.
丹红注射液对不稳定型心绞痛患者炎症因子、血栓素B2及血液流变学的影响.
