E03E0129 Mouse Epidermal Growth Factor Receptor ELISA kit
The Mouse Epidermal Growth Factor Receptor ELISA kit can be used to identify samples from the mouse species. Epidermal Growth Factor Receptor can also be called ErbB-1, EGFR, ERBB, ERBB1, HER1, NISBD2, PIG61, mENA, epidermal growth factor receptor, Genes, erbB-1, ERRP．
Product Information | |
Cat. No. | E03E0129 |
Product Name | Mouse Epidermal Growth Factor Receptor ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD E (0.5mL) | 5.0ng/ml | 1 vial |
STANDARD F (0.5mL) | 10 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
EGFR ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EGFR antibody and a EGFR-HRP conjugate. The assay sample and buffer are incubated together with EGFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EGFR concentration since EGFR from samples and EGFR-HRP conjugate compete for the anti-EGFR antibody binding site. Since the number of sites is limited, as more sites are occupied by EGFR from the sample, fewer sites are left to bind EGFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EGFR concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between EGFR and analogues was observed. | |
E03E0129 has been referenced in the below publications：
Influence of Electroacupuncture Changqiang(GV1)and Baihui(GV20)on SCN Output Signal Molecular of Mice Bearing Breast Cancer.
Related Bluegene Biotech Products
