Mouse Interleukin 6 ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Mouse Interleukin 6 ELISA kit

E03I0006 Mouse Interleukin 6 ELISA kit

The Mouse Interleukin 6 ELISA kit can be used to identify samples from the mouse species. Interleukin 6 can also be called Interleukin BSF 2, B cell differentiation factor, B cell stimulatory factor 2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor, Hepatocyte stimulating factor, HGF, HPGF, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL6 protein, Interferon beta 2, Interleukin 6 (interferon beta 2), Interleukin 6, IL 6.

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Specifications of Mouse Interleukin 6 ELISA kit

Product Information
Cat. No.	E03I0006
Product Name	Mouse Interleukin 6 ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	50-1000pg/ml
Sensitivity	1.0pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/ml	1 vial
STANDARD B (0.5mL)	50 pg/ml	1 vial
STANDARD C (0.5mL)	100 pg/ml	1 vial
STANDARD D (0.5mL)	250 pg/ml	1 vial
STANDARD E (0.5mL)	500 pg/ml	1 vial
STANDARD F (0.5mL)	1000 pg/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IL 6 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 6 antibody and an IL 6-HRP conjugate. The assay sample and buffer are incubated together with IL 6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 6 concentration since IL 6 from samples and IL 6-HRP conjugate compete for the anti-IL 6 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 6 from the sample, fewer sites are left to bind IL 6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 6 concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Interleukin 6 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IL 6 and analogues was observed.

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Summary of the Assay Procedure for Mouse Interleukin 6 ELISA kit

Citations of Mouse Interleukin 6 ELISA kit

E03I0006 has been referenced in the below publications：

Effect of lipid-bound apolipoprotein A-I cysteinemutant on ATF3 in RAW264.7 cells.

TLR9在细菌DNA诱导心肌炎症反应中BNP的作用。

Establishment of colitis-associated colon cancer mice model and the effects of annatto tocotrienol on peripheral T lymphocyte and cytokines.

THE INHIBITORY EFFECT OF APOLIPOPROTEIN AI MIMETIC PEPTIDE D-4F ON STEATOSIS AND INFLAMMATORY.

Effects of matrine combined with baicalin on mouse pneumonia induced by LPS.

Increased inflammatory reaction in tail-suspension mice infected by E. coli in spaceglight.

Effect of medium-chain fatty acid on the expression of TLＲ4 pathway related molecules in small intestine of C57BL/6J mice with obesity.

Effects of Ethanol Componentons from Aconitum vilmorinianum on Inflammatory Cytokines in LPS-stimulated RAW 264.7 Cell.

Protective effect of pioglitazone on lung injury induced by irradiation.

Lipopolysaccharide-Induced Preconditioning Attenuates Apoptosis and Differentially Regulates TLR4 and TLR7 Gene Expression after Ischemia in the Preterm Ovine Fetal Brain.

The Effects of Endurance Exercise and Adiponectin Receptor Agonist AdipoRon on Adipose Tissue Inflammation in High Fat Diet Mice.