E07G0036 Porcine Glucose 6 Phosphate ELISA kit
The Porcine Glucose 6 Phosphate ELISA kit can be used to identify samples from the porcine species. Glucose 6 Phosphate can also be called G6P, Robison ester.
Specifications of Porcine Glucose 6 Phosphate ELISA kit
Product Information | |
Cat. No. | E07G0036 |
Product Name | Porcine Glucose 6 Phosphate ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 umol/ml |
Sensitivity | 1.0 umol/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 umol/ml | 1 vial |
STANDARD B (0.5mL) | 5 umol/ml | 1 vial |
STANDARD C (0.5mL) | 10 umol/ml | 1 vial |
STANDARD D (0.5mL) | 25 umol/ml | 1 vial |
STANDARD E (0.5mL) | 50 umol/ml | 1 vial |
STANDARD F (0.5mL) | 100 umol/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
G6P ELISA kit uses an anti-G6P antibody and an G6P-HRP conjugate in a competitive enzyme immunoassay method. G6P-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-G6P antibody binding site between G6P from samples and G6P-HRP conjugate, the intensity of the color is inversely proportional to the concentration of G6P. Since the number of sites is limited, as more sites are occupied by G6P from the sample, fewer sites are left to bind G6P-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The G6P concentration in each sample is interpolated from this standard curve. |
Quality Control on Porcine Glucose 6 Phosphate ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between G6P and analogues was observed. | |
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Summary of the Assay Procedure for Porcine Glucose 6 Phosphate ELISA kit
Citations of Porcine Glucose 6 Phosphate ELISA kit
E07G0036 has been referenced in the below publications：
Correlation between three glycometabolic-related hormones and muscle glycolysis, as well as meat quality, in three pig breeds.
