E04G0157 Rabbit Glial Fibrillary Acidic Protein ELISA kit
Rabbit Glial Fibrillary Acidic Protein ELISA kit is suitable for detecting samples from rabbit species. Glial Fibrillary Acidic Protein can also be called GFAP, ALXDRD, glial fibrillary acidic protein.
Product Information | |
Cat. No. | E04G0157 |
Product Name | Rabbit Glial Fibrillary Acidic Protein ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000pg/ml |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GFAP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- GFAP antibody and an GFAP-HRP conjugate. The assay sample and buffer are incubated together with GFAP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GFAP concentration since GFAP from samples and GFAP-HRP conjugate compete for the anti-GFAP antibody binding site. Since the number of sites is limited, as more sites are occupied by GFAP from the sample, fewer sites are left to bind GFAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GFAP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GFAP and analogues was observed. | |
E04G0157 has been referenced in the below publications:
Intercostalarteries transplantion with bone marrow stromal cells for spinal cord injury.
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