E04H0124 Rabbit Heart Fatty Acid Binding Protein ELISA kit
Rabbit Heart Fatty Acid Binding Protein ELISA kit is suitable for detecting samples from rabbit species. Heart Fatty Acid Binding Protein can also be called hFABP, H-FABP, FABP3, FABP11, M-FABP, MDGI, O-FABP, Heart-type fatty acid binding protein, fatty acid binding protein 3, mammary-derived growth inhibitor, h FABP.
Product Information | |
Cat. No. | E04H0124 |
Product Name | Rabbit Heart Fatty Acid Binding Protein ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
h FABP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- h FABP antibody and an h FABP-HRP conjugate. The assay sample and buffer are incubated together with h FABP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the h FABP concentration since h FABP from samples and h FABP-HRP conjugate compete for the anti-h FABP antibody binding site. Since the number of sites is limited, as more sites are occupied by h FABP from the sample, fewer sites are left to bind h FABP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The h FABP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between h FABP and analogues was observed. | |
E04H0124 has been referenced in the below publications:
Serum H-FABP Content Changes After Myocardial Ischemia And Intervention Study Of Guizhi Soup Compound Prescription In New Zealand Rabbits.
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