E04L0022 Rabbit Low density lipoprotein cholesterol ELISA kit
Rabbit Low density lipoprotein cholesterol ELISA kit is suitable for detecting samples from rabbit species. Low density lipoprotein cholesterol can also be called LDL C, LDLC, LDL-C.
Specifications of Rabbit Low density lipoprotein cholesterol ELISA kit
Product Information | |
Cat. No. | E04L0022 |
Product Name | Rabbit Low density lipoprotein cholesterol ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LDL C ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- LDL C antibody and an LDL C-HRP conjugate. The assay sample and buffer are incubated together with LDL C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LDL C concentration since LDL C from samples and LDL C-HRP conjugate compete for the anti-LDL C antibody binding site. Since the number of sites is limited, as more sites are occupied by LDL C from the sample, fewer sites are left to bind LDL C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LDL C concentration in each sample is interpolated from this standard curve. |
Quality Control on Rabbit Low density lipoprotein cholesterol ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LDL C and analogues was observed. | |
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Summary of the Assay Procedure for Rabbit Low density lipoprotein cholesterol ELISA kit
Citations of Rabbit Low density lipoprotein cholesterol ELISA kit
E04L0022 has been referenced in the below publications:
To explore the inhibition of Polyinosinic acid combined statin on Cholesterol crystals as well as expression of hs-crp in atherosclerotic rabbits.
