E04B0203 Rabbit pro Brain Natriuretic Peptide ELISA kit
Rabbit pro Brain Natriuretic Peptide ELISA kit is suitable for detecting samples from rabbit species. pro Brain Natriuretic Peptide can also be called proBNP, BNP.
Specifications of Rabbit pro Brain Natriuretic Peptide ELISA kit
Product Information | |
Cat. No. | E04B0203 |
Product Name | Rabbit pro Brain Natriuretic Peptide ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
proBNP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- proBNP antibody and an proBNP-HRP conjugate. The assay sample and buffer are incubated together with proBNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the proBNP concentration since proBNP from samples and proBNP-HRP conjugate compete for the anti-proBNP antibody binding site. Since the number of sites is limited, as more sites are occupied by proBNP from the sample, fewer sites are left to bind proBNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The proBNP concentration in each sample is interpolated from this standard curve. |
Quality Control on Rabbit pro Brain Natriuretic Peptide ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between proBNP and analogues was observed. | |
BlueGene Biotech Product Show_copy20230504
Summary of the Assay Procedure for Rabbit pro Brain Natriuretic Peptide ELISA kit
Citations of Rabbit pro Brain Natriuretic Peptide ELISA kit
E04B0203 has been referenced in the below publications:
化瘀解毒法对急性肺血栓栓塞症兔D-二聚体、内皮素-1、B型脑利钠肽表达影响.
