E02M0204 Rat β2 Microglobulin ELISA kit
The Rat β2 Microglobulin ELISA kit can be used to identify samples from the rat species. β2 Microglobulin can also be called B2M, entrez:567, IMD43, beta-2-microglobulin, Β2 microglobulin, β2 MG.
Specifications of Rat β2 Microglobulin ELISA kit
Product Information | |
Cat. No. | E02M0204 |
Product Name | Rat β2 Microglobulin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ug/mL |
Sensitivity | 0.1 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ug/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ug/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD F (0.5mL) | 10 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
β2 MG ELISA kit uses an anti-β2 MG antibody and an β2 MG-HRP conjugate in a competitive enzyme immunoassay method. β2 MG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-β2 MG antibody binding site between β2 MG from samples and β2 MG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of β2 MG. Since the number of sites is limited, as more sites are occupied by β2 MG from the sample, fewer sites are left to bind β2 MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The β2 MG concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat β2 Microglobulin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between β2 MG and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat β2 Microglobulin ELISA kit
Citations of Rat β2 Microglobulin ELISA kit
E02M0204 has been referenced in the below publications：
电针刺激“足三里”、“曲池”穴对自发性高血压大鼠血压和肾功能影响的机制研究。
