E02F0007 Rat Fibrinogen ELISA kit
The Rat Fibrinogen ELISA kit can be used to identify samples from the rat species. Fibrinogen can also be called F1, FI, Fbg, Coagulation Factor I, Fb.
Specifications of Rat Fibrinogen ELISA kit
Product Information | |
Cat. No. | E02F0007 |
Product Name | Rat Fibrinogen ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Fb ELISA kit uses an anti-Fb antibody and an Fb-HRP conjugate in a competitive enzyme immunoassay method. Fb-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Fb antibody binding site between Fb from samples and Fb-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Fb. Since the number of sites is limited, as more sites are occupied by Fb from the sample, fewer sites are left to bind Fb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Fb concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Fibrinogen ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Fb and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Fibrinogen ELISA kit
Citations of Rat Fibrinogen ELISA kit
E02F0007 has been referenced in the below publications：
Study on the protective effects of Qishen Yiqi pill on myocardial ischemia reperfusion injury in rats.
Influence of Endothelial Function and Blood Ｒheology in Diabetic Ｒats by Paeonol and Tanshinol.
Pharmacokinetics, tissue distribution and excretion of porcine fibrinogen after intraperitoneal injection of a porcine-derived fibrin glue to rats.
