E02M0023 Rat Malondialdehyde ELISA kit
The Rat Malondialdehyde ELISA kit can be used to identify samples from the rat species. Malondialdehyde can also be called MDA.
Specifications of Rat Malondialdehyde ELISA kit
Product Information | |
Cat. No. | E02M0023 |
Product Name | Carcinoembryonic |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 10 ng/ml | 1 vial |
STANDARD D (0.5mL) | 25 ng/ml | 1 vial |
STANDARD E (0.5mL) | 50 ng/ml | 1 vial |
STANDARD F (0.5mL) | 100 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MDA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MDA. Standards or samples are then added to the microtiter plate wells and MDA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MDA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MDA are added to each well to “sandwich” the MDA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MDA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MDA concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Malondialdehyde ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MDA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Malondialdehyde ELISA kit
Citations of Rat Malondialdehyde ELISA kit
E02M0023 has been referenced in the below publications：
Functional status of microvascular vasomotion is impaired in spontaneously hypertensive rat.
Curcumin prevents the non-alcoholic fatty hepatitis via mitochondria protection and apoptosis reduction.
Effect of Huatanzhuyu decoction on MDA, SOD, β-APPmRNA in hippocampus in rat models of Alzheimer’s disease.
Effects of Loaded Swimming Exercise on Knee Osteoarthritis in Rats.
The experimental and clinical Research Based on the Theory of "Xuan Fu" Explore Yizhi Tongxuan Decoction for the Treatment of Senile Dementia Mechanism.
Liver injury attenuation by curcumin in a rat NASH model: an Nrf2 activation-mediated effect?.
Pancreatic Microcirculation Profiles in the Progression of Hypertension in Spontaneously Hypertensive Rats.
Comparison of Pancreatic Microcirculation Profiles in Spontaneously Hypertensive Rats and Wistar-Kyoto Rats by Laser Doppler and Wavelet Transform Analysis.
