Rat Total Adiponectin ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Rat Total Adiponectin ELISA kit

E02A0125 Rat Total Adiponectin ELISA kit

The Rat Total Adiponectin ELISA kit can be used to identify samples from the rat species. Total Adiponectin can also be called 30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP 30, ACRP30, ADIPO, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, Adiponectin, AdipoQ, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPQTL1, ADPN, APM 1, apM-1, ApM1, C1q and collagen domain-containing protein, GBP 28, GBP28, Gelatin binding protein, Gelatin binding protein 28, Gelatin-binding protein.

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Specifications of Rat Total Adiponectin ELISA kit

Product Information
Cat. No.	E02A0125
Product Name	Rat Total Adiponectin ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	2.5-50 ng/mL
Sensitivity	0.1 ng/ml
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	2.5 ng/ml	1 vial
STANDARD C (0.5mL)	5.0 ng/ml	1 vial
STANDARD D (0.5mL)	10 ng/ml	1 vial
STANDARD E (0.5mL)	25 ng/ml	1 vial
STANDARD F (0.5mL)	50 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

ADPN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ADPN. Standards or samples are then added to the microtiter plate wells and ADPN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADPN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADPN are added to each well to “sandwich” the ADPN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ADPN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADPN concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Total Adiponectin ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between ADPN and analogues was observed.

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Citations of Rat Total Adiponectin ELISA kit

E02A0125 has been referenced in the below publications：

麝香保心丸干预模型大鼠急性心肌梗死后心室重构的机理研究。

Discussion on Effects of Different Aerobic Exercise Intensities on the Hepatitis of NAFLD Rats Based on the SIRT1 Axis.

Effect of QizhiJiangtang Capsule on insulin resistance in diabetic rats and its mechanism.

Role of microecologics in the progression of nonalcoholic steatohepatitis.

Protective effects and mechanism of matrine on high-fructose-diet induced non-alcoholic fatty liver in rats.

Treatment Pioglitazone induces regression and stabilization of coronary atherosclerotic plaques in patients with impaired glucose tolerance.