Mouse Microtubule Associated Proteins 1A/1B Light Chain 3B ELISA kit (E03M0531)

BlueGene Biotech Mouse Microtubule Associated Proteins 1A/1B Light Chain 3B ELISA kit (E03M0531)

E03M0531 Mouse Microtubule Associated Proteins 1A/1B Light Chain 3B ELISA kit

The Mouse Microtubule Associated Proteins 1A/1B Light Chain 3B ELISA kit can be used to identify samples from the mouse species. Microtubule Associated Proteins 1A/1B Light Chain 3B can also be called Microtubule-associated protein 1 light chain 3 beta, ATG8F, Autophagy related protein LC3 B, Autophagy related ubiquitin like modifier LC3 B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3 like protein 2, MAP1 light chain 3-like protein 2, MAP1A/1B light chain 3 B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, LC3B.

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Specifications of Mouse Microtubule Associated Proteins 1A/1B Light Chain 3B ELISA kit

Product Information
Cat. No.	E03M0531
Product Name	Mouse Microtubule Associated Proteins 1A/1B Light Chain 3B ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	50-1000pg/ml
Sensitivity	1.0pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/ml	1 vial
STANDARD B (0.5mL)	50 pg/ml	1 vial
STANDARD C (0.5mL)	100 pg/ml	1 vial
STANDARD D (0.5mL)	250 pg/ml	1 vial
STANDARD E (0.5mL)	500 pg/ml	1 vial
STANDARD F (0.5mL)	1000 pg/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

LC3B ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LC3B antibody and an LC3B-HRP conjugate. The assay sample and buffer are incubated together with LC3B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LC3B concentration since LC3B from samples and LC3B-HRP conjugate compete for the anti-LC3B antibody binding site. Since the number of sites is limited, as more sites are occupied by LC3B from the sample, fewer sites are left to bind LC3B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LC3B concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Microtubule Associated Proteins 1A/1B Light Chain 3B ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between LC3B and analogues was observed.

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Summary of the Assay Procedure for Mouse Microtubule Associated Proteins 1A/1B Light Chain 3B ELISA kit

Citations of Mouse Microtubule Associated Proteins 1A/1B Light Chain 3B ELISA kit

E03M0531 has been referenced in the below publications：

The suppressed autophagy induced by carbon disulfide could be rescued by N-carbamoyl glutamate during the window of embryo implantation in mice.