E02E0062 Rat Extracellular signal Regulated Kinase1/2 ELISA kit
The Rat Extracellular signal Regulated Kinase1/2 ELISA kit can be used to identify samples from the rat species. Extracellular signal Regulated Kinase1/2 can also be called ERK1/2.
Specifications of Rat Extracellular signal Regulated Kinase1/2 ELISA kit
Product Information | |
Cat. No. | E02E0062 |
Product Name | Rat Extracellular signal Regulated Kinase1/2 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ERK1/2 ELISA kit uses an anti-ERK1/2 antibody and an ERK1/2-HRP conjugate in a competitive enzyme immunoassay method. ERK1/2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ERK1/2 antibody binding site between ERK1/2 from samples and ERK1/2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ERK1/2. Since the number of sites is limited, as more sites are occupied by ERK1/2 from the sample, fewer sites are left to bind ERK1/2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ERK1/2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Extracellular signal Regulated Kinase1/2 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ERK1/2 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Extracellular signal Regulated Kinase1/2 ELISA kit
Citations of Rat Extracellular signal Regulated Kinase1/2 ELISA kit
E02E0062 has been referenced in the below publications：
Nonalcoholic fatty liver tissue of hepatitis TNFΑ, TGFΒ1, LEPTIN and MAPK expression and drug intervention.
Experimental study of calmodulin-dependent protein kinase II and extracellular signal regulated kinase on the impact of erectile dysfunction in rats with hyperlipidemia.
