E01S0076 Human Stromal Cell Derived Factor 4/45 kDa calcium binding protein ELISA kit
The Human Stromal Cell Derived Factor 4/45 kDa calcium binding protein ELISA kit can be used to identify samples from the human species. Stromal Cell Derived Factor 4/45 kDa calcium binding protein can also be called SDF4, Cab45, SDF-4, stromal cell derived factor 4.
Product Information | |
Cat. No. | E01S0076 |
Product Name | Human Stromal Cell Derived Factor 4/45 kDa calcium binding protein ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SDF4/Cab45 ELISA kit uses an anti-SDF4/Cab45 antibody and an SDF4/Cab45-HRP conjugate in a competitive enzyme immunoassay method. SDF4/Cab45-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SDF4/Cab45 antibody binding site between SDF4/Cab45 from samples and SDF4/Cab45-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SDF4/Cab45. Since the number of sites is limited, as more sites are occupied by SDF4/Cab45 from the sample, fewer sites are left to bind SDF4/Cab45-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF4/Cab45 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SDF4/Cab45 and analogues was observed. | |
E01S0076 has been referenced in the below publications：
A Novel Role of Cab45-G in Mediating Cell Migration in Cancer Cells.
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