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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Stromal Cell Derived Factor 4/45 kDa calcium binding protein ELISA kit (E01S0076)

E01S0076 Human Stromal Cell Derived Factor 4/45 kDa calcium binding protein ELISA kit

The Human Stromal Cell Derived Factor 4/45 kDa calcium binding protein ELISA kit can be used to identify samples from the human species. Stromal Cell Derived Factor 4/45 kDa calcium binding protein can also be called SDF4, Cab45, SDF-4, stromal cell derived factor 4.

Products

Specifications of Human Stromal Cell Derived Factor 4/45 kDa calcium binding protein ELISA kit

Product Information

Cat. No.

E01S0076

Product Name

Human Stromal Cell Derived Factor 4/45 kDa calcium binding protein ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SDF4/Cab45 ELISA kit uses an anti-SDF4/Cab45 antibody and an SDF4/Cab45-HRP conjugate in a competitive enzyme immunoassay method. SDF4/Cab45-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SDF4/Cab45 antibody binding site between SDF4/Cab45 from samples and SDF4/Cab45-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SDF4/Cab45. Since the number of sites is limited, as more sites are occupied by SDF4/Cab45 from the sample, fewer sites are left to bind SDF4/Cab45-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF4/Cab45 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Stromal Cell Derived Factor 4/45 kDa calcium binding protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SDF4/Cab45 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Stromal Cell Derived Factor 4/45 kDa calcium binding protein ELISA kit

Summary of the Assay Procedure for Human Stromal Cell Derived Factor 4/45 kDa calcium binding protein ELISA kit

Citations of Human Stromal Cell Derived Factor 4/45 kDa calcium binding protein ELISA kit

E01S0076 has been referenced in the below publications:

A Novel Role of Cab45-G in Mediating Cell Migration in Cancer Cells.

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