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BlueGene Biotech Mouse Serotonin/5 Hydroxytryptamine ELISA kit (E03H0106)

E03H0106 Mouse Serotonin/5 Hydroxytryptamine ELISA kit

The Mouse Serotonin/5 Hydroxytryptamine ELISA kit can be used to identify samples from the mouse species. Serotonin/5 Hydroxytryptamine can also be called 5HT, Serotonin, N-Methyl-Gamma.

Products

Specifications of Mouse Serotonin/5 Hydroxytryptamine ELISA kit

Product Information

Cat. No.

E03H0106

Product Name

Mouse Serotonin/5 Hydroxytryptamine ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

5.0-100ng/ml

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

5 HT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-5 HT antibody and an 5 HT-HRP conjugate. The assay sample and buffer are incubated together with 5 HT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 5 HT concentration since 5 HT from samples and 5 HT-HRP conjugate compete for the anti-5 HT antibody binding site. Since the number of sites is limited, as more sites are occupied by 5 HT from the sample, fewer sites are left to bind 5 HT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 5 HT concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Serotonin/5 Hydroxytryptamine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 5 HT and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Serotonin/5 Hydroxytryptamine ELISA kit

Summary of the Assay Procedure for Mouse Serotonin/5 Hydroxytryptamine ELISA kit

Citations of Mouse Serotonin/5 Hydroxytryptamine ELISA kit

E03H0106 has been referenced in the below publications:

Effects of chronic noise exposure on the microbiome-gut-brain axis in senescence-accelerated prone mice:implications for Alzheimer’s disease.

Study on the mice neurotoxicity of butyl benzyl phthalate in vivo and in vitro.

The effects of cyanidin-3-glucoside on platelet granule secretion in hypercholesterolemia mice.

归脾汤对苯中毒小鼠外周血指标及脑组织中5-TH、DA的影响。

Environmental noise stress disturbs commensal microbiota homeostasis and induces oxi-inflammmation and AD-like neuropathology through epithelial barrier disruption in the EOAD mouse model.

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