Rat Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit (E02R0349)

BlueGene Biotech Rat Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit (E02R0349)

E02R0349 Rat Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

The Rat Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit can be used to identify samples from the rat species. Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor can also be called Advanced glycosylation end product specific receptor, Advanced glycosylation end product-specific receptor, AGER, LE 9211 A antigen,LE-9211-A antigen, MOK, RAGE 1, RAGE1, MOK protein kinase, Receptor for advanced glycation endproducts, Renal tumor antigen 1, Renal tumor antigen, Renal cell carcinoma antigen (MOK protein kinase), Renal tumor antigen 1.

Products

Specifications of Rat Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

Product Information
Cat. No.	E02R0349
Product Name	Rat Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/ml
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

RAGE ELISA kit uses an anti-RAGE antibody and an RAGE-HRP conjugate in a competitive enzyme immunoassay method. RAGE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-RAGE antibody binding site between RAGE from samples and RAGE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of RAGE. Since the number of sites is limited, as more sites are occupied by RAGE from the sample, fewer sites are left to bind RAGE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RAGE concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between RAGE and analogues was observed.

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Summary of the Assay Procedure for Rat Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

Citations of Rat Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

E02R0349 has been referenced in the below publications：

Influence of different resuscitation strategies on SIOOB and receptor of advanced glycation end—products(RAGE)in rat brain tissue after hemorrhagic shock.