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BlueGene Biotech Human 14-3-3 protein beta/alpha ELISA kit (E01Y0004)

E01Y0004 Human 14-3-3 protein beta/alpha ELISA kit

The Human 14-3-3 protein beta/alpha ELISA kit can be used to identify samples from the human species. 14-3-3 protein beta/alpha can also be called YWHAB, GW128, HEL-S-1, HS1, KCIP-1, YWHAA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta.

Products

Specifications of Human 14-3-3 protein beta/alpha ELISA kit

Product Information

Cat. No.

E01Y0004

Product Name

Human 14-3-3 protein beta/alpha ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

YWHAB ELISA kit uses an anti-YWHAB antibody and an YWHAB-HRP conjugate in a competitive enzyme immunoassay method. YWHAB-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-YWHAB antibody binding site between YWHAB from samples and YWHAB-HRP conjugate, the intensity of the color is inversely proportional to the concentration of YWHAB. Since the number of sites is limited, as more sites are occupied by YWHAB from the sample, fewer sites are left to bind YWHAB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YWHAB concentration in each sample is interpolated from this standard curve.


Quality Control on Human 14-3-3 protein beta/alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between YWHAB and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human 14-3-3 protein beta/alpha ELISA kit

Summary of the Assay Procedure for Human 14-3-3 protein beta/alpha ELISA kit

Citations of Human 14-3-3 protein beta/alpha ELISA kit

E01Y0004 has been referenced in the below publications:

Cellular stress and redox activity proteins are involved in gastric carcinogenesis associated with Helicobacter pylori infection expressing high levels of thioredoxin-1.

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