EH-E0020-1-Ab Anti-E.coli Host Cell Protein G3 Antibody has been validated by ELISA and 2D-WB methods, demonstrating high affinity for HCP, broad HCP coverage, and E.coli cell specificity. It can be used for bridging experiments or quality control testing during production.
Specifications Of EH-E0020-1-Ab Anti-E.coli Host Cell Protein G3 Antibody
| Product Information | |
Catalog Number | EH-E0020-1-Ab |
Package Size | 100μg |
| Type | Sheep polyclonal antibody Ig |
| Reactive Species | E.coli |
| Purity | ≥95% |
| Physical Property | Liquid |
| Components | 0.01M PBS+50% glycerol, 0.3‰ Prolin300, sterile solution |
| Storage | -20℃, Aliquoting is recommended for storage |
| Applications | ELISA, WB, Low-adsorption, High-throughput Protein Chip System, LC-MS |
| Coverage | ≥70% (validated by 2D-WB method) |
| Shelf Life | 5 Years |
Product Introduction |
E.coli cell lines are widely used as an expression system. During the process of cell disruption to extract the target protein, a large proportion of host proteins are released together with the target protein., many of which have strong immunogenicity. This can lead to adverse toxicity or immune reactions, jeopardizing the safety and quality of the product and potentially causing biological contamination. One of the objectives of downstream processes in biopharmaceutical production is to remove these potential hazards. Therefore, it is essential to minimize the residual amount of host cell protein to the lowest levels. When developing downstream purification processes, a scientifically sound and rational method for qualitatively assessing HCP in the final or semi-product must be in place. This antibody has been validated by ELISA and 2D-WB methods, demonstrating high affinity for HCP, broad HCP coverage, and E.coli cell specificity. It can be used for bridging experiments or quality control testing during production. |
