PH-E0021-1-Ab Anti-Pichia Yeast Host Cell Protein G2 Antibody has been validated by ELISA and 2D-WB methods, demonstrating high affinity for HCP, broad HCP coverage, and Pichia Yeast cell specificity. It can be used for bridging experiments or quality control testing during production.
Specifications Of PH-E0021-1-Ab Anti-Pichia Yeast Host Cell Protein G2 Antibody
| Product Information | |
Catalog Number | PH-E0021-1-Ab |
Package Size | 100μg |
| Type | Rabbit polyclonal antibody IgG |
| Reactive Species | Pichia Yeast |
| Purity | ≥95% |
| Physical Property | Liquid |
| Components | 0.01M PBS+50% glycerol, 0.3‰ Prolin300, sterile solution |
| Storage | -20℃, Aliquoting is recommended for storage |
| Applications | ELISA, WB, Low-adsorption, High-throughput Protein Chip System, LC-MS |
| Coverage | ≥70% (validated by 2D-WB method) |
| Shelf Life | 5 Years |
Product Introduction |
Pichia Yeast cell lines, as a commonly used expression system, undergoes apoptosis during the expression of target proteins. Studies have shown that after cell disruption, host proteins released into the culture medium can amount to more than a thousand different types, many of which have strong immunogenicity. This can lead to adverse toxicity or immune reactions, jeopardizing the safety and quality of the product and potentially causing biological contamination. One of the objectives of downstream processes in biopharmaceutical production is to remove these potential hazards. Therefore, it is essential to minimize the residual amount of host cell protein to the lowest levels. When developing downstream purification processes, a scientifically sound and rational method for qualitatively assessing HCP in the final or semi-product must be in place. This antibody has been validated by ELISA and 2D-WB methods, demonstrating high affinity for HCP, broad HCP coverage, and Pichia Yeast cell specificity. It can be used for bridging experiments or quality control testing during production. |
