E02C1217 Rat coiled coil myosin like BCL2 interacting protein/Beclin 1 ELISA kit
The Rat coiled coil myosin like BCL2 interacting protein/Beclin 1 ELISA kit can be used to identify samples from the rat species. coiled coil myosin like BCL2 interacting protein/Beclin 1 can also be called BECN1, ATG6, VPS30, beclin1, beclin 1.
Product Information | |
Cat. No. | E02C1217 |
Product Name | Rat coiled coil myosin like BCL2 interacting protein/Beclin 1 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
BECN1 ELISA kit uses an anti-BECN1 antibody and an BECN1-HRP conjugate in a competitive enzyme immunoassay method. BECN1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-BECN1 antibody binding site between BECN1 from samples and BECN1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of BECN1. Since the number of sites is limited, as more sites are occupied by BECN1 from the sample, fewer sites are left to bind BECN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BECN1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between BECN1 and analogues was observed. | |
E02C1217 has been referenced in the below publications：
The Change of Autophagy in Mice after Exposed to Carbon Disulfide at Peri-implantation and the Effect on the Pregnancy Outcome after Mice Diet Supplement NCG.
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