E11H1420 Bovine 3-Hydroxybutyric acid ELISA Kit
The Bovine 3-Hydroxybutyric acid ELISA Kit can be used to identify samples from the bovine species. 3-Hydroxybutyric acid can also be called OHB, BHBA, Beta-Hydroxybutyrate, 3-Hydroxybutyric Acid, β-Hydroxybutyric acid, HYA.
Product Information | |
Cat. No. | E11H1420 |
Product Name | Bovine 3-Hydroxybutyric acid ELISA Kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 umol/L |
Sensitivity | 0.1 umol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 umol/L | 1 vial |
STANDARD B (0.5mL) | 0.5 umol/L | 1 vial |
STANDARD C (0.5mL) | 1.0 umol/L | 1 vial |
STANDARD D (0.5mL) | 2.5 umol/L | 1 vial |
STANDARD E (0.5mL) | 5.0 umol/L | 1 vial |
STANDARD F (0.5mL) | 10 umol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HYA ELISA kit uses an anti-HYA antibody and an HYA-HRP conjugate in a competitive enzyme immunoassay method. HYA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HYA antibody binding site between HYA from samples and HYA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HYA. Since the number of sites is limited, as more sites are occupied by HYA from the sample, fewer sites are left to bind HYA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HYA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HYA and analogues was observed. | |
E11H1420 has been referenced in the below publications：
Effect of Different Feeding Patterns on Serum Biochemical Indices and Hormone Content in Dairy Cows.
Pathway analysis of plasma different metabolites for dairy cow ketosis.
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