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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Acetyl CoA Carboxylase 1 ELISA kit (E11A1169)

E11A1169 Bovine Acetyl CoA Carboxylase 1 ELISA kit

Bovine Acetyl CoA carboxylase 1 ELISA kit is suitable for the detection of samples from Bovine species. Acetyl CoA carboxylase 1 can also be called ACAC, ACC1, ACCA, ACAC, ACC2, ACCB, ACACB.

Products

Specifications of Bovine Acetyl CoA Carboxylase 1 ELISA kit

Product Information

Cat. No.

E11A1169

Product Name

Bovine Acetyl CoA carboxylase 1 ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C



Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

The competitive enzyme immunoassay method is used by the ACACA ELISA kit in conjunction with an anti-ACACA antibody and an ACACA-HRP conjugate. In a pre-coated plate, the test sample, buffer, and ACACA-HRP conjugate are incubated for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ACACA antibody binding site between ACACA from samples and ACACA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ACACA. As more sites are filled by ACACA from the sample, fewer sites are left to bind ACACA-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration.


Quality Control on Bovine Acetyl CoA Carboxylase 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

90-102%

Linearity

Diluent Ratio

Range %

1:2

87-105

1:4

84-109

1:8

85-107

Specificity/ Cross-reactivity

No significant cross-reactivity or interference between ACACA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Acetyl CoA Carboxylase 1 ELISA kit

Summary of the Assay Procedure for Bovine Acetyl CoA Carboxylase 1 ELISA kit

Citations of Bovine Acetyl CoA Carboxylase 1 ELISA kit

E11A1169 has been referenced in the below publications:

Non-Esterified Fatty Acids Activate the AMP-Activated Protein Kinase Signaling Pathway to Regulate Lipid Metabolism in Bovine Hepatocytes.

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