E11S0035 Bovine Alpha Synuclein oligomer ELISA kit
The Bovine Alpha Synuclein oligomer ELISA kit can be used to identify samples from the bovine species. Alpha Synuclein oligomer can also be called NACP, PARK1, PARK4, PD1, Alpha SYNo, αSYNo, α-SYNo.
Specifications of Bovine Alpha Synuclein oligomer ELISA kit
Product Information | |
Cat. No. | E11S0035 |
Product Name | Bovine Alpha Synuclein oligomer ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Alpha SYNo ELISA kit uses an anti-Alpha SYNo antibody and an Alpha SYNo-HRP conjugate in a competitive enzyme immunoassay method. Alpha SYNo-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Alpha SYNo antibody binding site between Alpha SYNo from samples and Alpha SYNo-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Alpha SYNo. Since the number of sites is limited, as more sites are occupied by Alpha SYNo from the sample, fewer sites are left to bind Alpha SYNo-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Alpha SYNo concentration in each sample is interpolated from this standard curve. |
Quality Control on Bovine Alpha Synuclein oligomer ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Alpha SYNo and analogues was observed. | |
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Summary of the Assay Procedure for Bovine Alpha Synuclein oligomer ELISA kit
Citations of Bovine Alpha Synuclein oligomer ELISA kit
E11S0035 has been referenced in the below publications：
Proteasome activity regulated by charged gold nanoclusters: Implications for neurodegenerative diseases.
