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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Alpha Synuclein oligomer ELISA kit (E11S0035)

E11S0035 Bovine Alpha Synuclein oligomer ELISA kit

The Bovine Alpha Synuclein oligomer ELISA kit can be used to identify samples from the bovine species. Alpha Synuclein oligomer can also be called NACP, PARK1, PARK4, PD1, Alpha  SYNo, αSYNo, α-SYNo.

Products

Specifications of Bovine Alpha Synuclein oligomer ELISA kit

Product Information

Cat. No.

E11S0035

Product Name

Bovine Alpha Synuclein oligomer ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Alpha  SYNo ELISA kit uses an anti-Alpha  SYNo antibody and an Alpha  SYNo-HRP conjugate in a competitive enzyme immunoassay method. Alpha  SYNo-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Alpha  SYNo antibody binding site between Alpha  SYNo from samples and Alpha  SYNo-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Alpha  SYNo. Since the number of sites is limited, as more sites are occupied by Alpha  SYNo from the sample, fewer sites are left to bind Alpha  SYNo-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Alpha  SYNo concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Alpha Synuclein oligomer ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Alpha  SYNo and analogues was observed.


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Summary of the Assay Procedure for Bovine Alpha Synuclein oligomer ELISA kit

Summary of the Assay Procedure for Bovine Alpha Synuclein oligomer ELISA kit

Citations of Bovine Alpha Synuclein oligomer ELISA kit

E11S0035 has been referenced in the below publications:

Proteasome activity regulated by charged gold nanoclusters: Implications for neurodegenerative diseases.

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