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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Chemerin ELISA kit (E11C0104)

E11C0104 Bovine Chemerin ELISA kit

Bovine Chemerin ELISA kit is suitable for the detection of samples from Bovine species. Chemerin can also be called TIG2, RARRES2, Tazarotene-Induced Gene 2, CHE.

Products

Specifications of Bovine Chemerin ELISA kit

Product Information

Cat. No.

E11C0104

Product Name

Bovine Chemerin ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

5-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CHE ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CHE antibody and an CHE-HRP conjugate. The assay sample and buffer are incubated together with CHE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHE concentration since CHE from samples and CHE-HRP conjugate compete for the anti-CHE antibody binding site. Since the number of sites is limited, as more sites are occupied by CHE from the sample, fewer sites are left to bind CHE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHE concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Chemerin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-102%

Linearity

Diluent Ratio

Range %

1:2

90-104

1:4

85-105

1:8

82-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CHE and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Chemerin ELISA kit

Summary of the Assay Procedure for Bovine Chemerin ELISA kit

Citations of Bovine Chemerin ELISA kit

E11C0104 has been referenced in the below publications:

Adipokines as biomarkers of postpartum subclinical endometritis in dairy cows.

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