E11C0376 Bovine Cholecystokinin ELISA kit
The Bovine Cholecystokinin ELISA kit can be used to identify samples from the bovine species. Cholecystokinin can also be called CCK, CCK-PZ.
Specifications of Bovine Cholecystokinin ELISA kit
Product Information | |
Cat. No. | E11C0376 |
Product Name | Bovine Cholecystokinin ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CCK ELISA kit uses an anti-CCK antibody and an CCK-HRP conjugate in a competitive enzyme immunoassay method. CCK-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CCK antibody binding site between CCK from samples and CCK-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CCK. Since the number of sites is limited, as more sites are occupied by CCK from the sample, fewer sites are left to bind CCK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCK concentration in each sample is interpolated from this standard curve. |
Quality Control on Bovine Cholecystokinin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CCK and analogues was observed. | |
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Summary of the Assay Procedure for Bovine Cholecystokinin ELISA kit
Citations of Bovine Cholecystokinin ELISA kit
E11C0376 has been referenced in the below publications：
Associations of blood parameters with age, feed efficiency and sampling routine in young beef bulls.
