E11G0201 Bovine Glucose Transporter 4 ELISA kit
The Bovine Glucose Transporter 4 ELISA kit can be used to identify samples from the bovine species. Glucose Transporter 4 can also be called ALB; Serum albumin.
Specifications of Bovine Glucose Transporter 4 ELISA kit
Product Information | |
Cat. No. | E11G0201 |
Product Name | Bovine Glucose Transporter 4 ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GLUT4 ELISA kit uses an anti-GLUT4 antibody and an GLUT4-HRP conjugate in a competitive enzyme immunoassay method. GLUT4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GLUT4 antibody binding site between GLUT4 from samples and GLUT4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GLUT4. Since the number of sites is limited, as more sites are occupied by GLUT4 from the sample, fewer sites are left to bind GLUT4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLUT4 concentration in each sample is interpolated from this standard curve. |
Quality Control on Bovine Glucose Transporter 4 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GLUT4 and analogues was observed. | |
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Summary of the Assay Procedure for Bovine Glucose Transporter 4 ELISA kit
Citations of Bovine Glucose Transporter 4 ELISA kit
E11G0201 has been referenced in the below publications：
Impact of curcumin on insulin resistant and infammatory in nonalcoholic steatohepatitis cell model.
