E11G0369 Bovine Glutathione peroxidase ELISA kit
The Bovine Glutathione peroxidase ELISA kit can be used to identify samples from the bovine species. Glutathione peroxidase can also be called GPx, GSHPx.
Specifications of Bovine Glutathione peroxidase ELISA kit
Product Information | |
Cat. No. | E11G0369 |
Product Name | Bovine Glutathione peroxidase ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GSH Px ELISA kit uses an anti-GSH Px antibody and an GSH Px-HRP conjugate in a competitive enzyme immunoassay method. GSH Px-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GSH Px antibody binding site between GSH Px from samples and GSH Px-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GSH Px. Since the number of sites is limited, as more sites are occupied by GSH Px from the sample, fewer sites are left to bind GSH Px-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSH Px concentration in each sample is interpolated from this standard curve. |
Quality Control on Bovine Glutathione peroxidase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GSH Px and analogues was observed. | |
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Summary of the Assay Procedure for Bovine Glutathione peroxidase ELISA kit
Citations of Bovine Glutathione peroxidase ELISA kit
E11G0369 has been referenced in the below publications：
Low Expression of Sirtuin 1 in the Dairy Bovines with Mild Fatty Liver Alters Hepatic Lipid Metabolism.
