E11H0033 Bovine Hydroxylysyl Pyridinoline ELISA kit
The Bovine Hydroxylysyl Pyridinoline ELISA kit can be used to identify samples from the bovine species. Hydroxylysyl Pyridinoline can also be called HP, Pyridinoline, PYD.
Specifications of Bovine Hydroxylysyl Pyridinoline ELISA kit
Product Information | |
Cat. No. | E11A0028 |
Product Name | Bovine Hydroxylysyl Pyridinoline ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 nmol/L |
Sensitivity | 1.0 nmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 nmol/L | 1 vial |
STANDARD B (0.5mL) | 10 nmol/L | 1 vial |
STANDARD C (0.5mL) | 25 nmol/L | 1 vial |
STANDARD D (0.5mL) | 50 nmol/L | 1 vial |
STANDARD E (0.5mL) | 100 nmol/L | 1 vial |
STANDARD F (0.5mL) | 250 nmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HP ELISA kit uses an anti-HP antibody and an HP-HRP conjugate in a competitive enzyme immunoassay method. HP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HP antibody binding site between HP from samples and HP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HP. Since the number of sites is limited, as more sites are occupied by HP from the sample, fewer sites are left to bind HP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HP concentration in each sample is interpolated from this standard curve. |
Quality Control on Bovine Hydroxylysyl Pyridinoline ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HP and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Bovine Hydroxylysyl Pyridinoline ELISA kit
Citations of Bovine Hydroxylysyl Pyridinoline ELISA kit
E11A0028 has been referenced in the below publications：
Effect of Different Feeding Patterns on Serum Biochemical Indices and Hormone Content in Dairy Cows.
