E01S0014 Human Serum Albumin ELISA kit
The Human Serum Albumin ELISA kit can be used to identify samples from the human species. Serum Albumin can also be called Albumin, Fraction V.
Specifications of Human Serum Albumin ELISA kit
Product Information | |
Cat. No. | E01S0014 |
Product Name | Human Serum Albumin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 100 ng/mL | 1 vial |
STANDARD C (0.5mL) | 250 ng/mL | 1 vial |
STANDARD D (0.5mL) | 500 ng/mL | 1 vial |
STANDARD E (0.5mL) | 1000 ng/mL | 1 vial |
STANDARD F (0.5mL) | 2500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SALB ELISA kit uses an anti-SALB antibody and an SALB-HRP conjugate in a competitive enzyme immunoassay method. SALB-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SALB antibody binding site between SALB from samples and SALB-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SALB. Since the number of sites is limited, as more sites are occupied by SALB from the sample, fewer sites are left to bind SALB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SALB concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Serum Albumin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SALB and analogues was observed. | |
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Summary of the Assay Procedure for Human Serum Albumin ELISA kit
Citations of Human Serum Albumin ELISA kit
E01S0014 has been referenced in the below publications：
Enhanced liver functions of HepG2 cells in the alginate/xyloglucan scaffold.
