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BlueGene Biotech Human Malondialdehyde ELISA kit (E01M0023)

E11I0308 Human Malondialdehyde ELISA kit

The Human Malondialdehyde ELISA kit can be used to identify samples from the human species. Malondialdehyde can also be called Malondialdehyde MDA.

Products

Specifications of Human Malondialdehyde ELISA kit

Product Information

Cat. No.

E01M0023

Product Name

Human Malondialdehyde ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

100-2500 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

100 ng/mL

1 vial

STANDARD C (0.5mL)

250 ng/mL

1 vial

STANDARD D (0.5mL)

500 ng/mL

1 vial

STANDARD E (0.5mL)

1000 ng/mL

1 vial

STANDARD F (0.5mL)

2500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MDA ELISA kit uses an anti-MDA antibody and an MDA-HRP conjugate in a competitive enzyme immunoassay method. MDA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MDA antibody binding site between MDA from samples and MDA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MDA. Since the number of sites is limited, as more sites are occupied by MDA from the sample, fewer sites are left to bind MDA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MDA concentration in each sample is interpolated from this standard curve.


Quality Control on Human Malondialdehyde ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MDA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Malondialdehyde ELISA kit

Summary of the Assay Procedure for Human Malondialdehyde ELISA kit

Citations of Human Malondialdehyde ELISA kit

E01M0023 has been referenced in the below publications:

The changes of mitochondrial DNA levels in peripheral blood monouclear cells in patients with chronic hepatitis b receiving long- term adefovir treatment.

The Clinical Study on the effect of Kun Fu Kang Capsule on Red Cell Immunology with Chronic Pelvic Inflammatory Disease.

A cell model of nonalcoholic steatohepatitis induced by FFA: establishment and dynamic monitoring.

Entecavir treatment causes injury to the mitochondrial DNA of peripheral blood mononudear cells.

Effects of rapid entrance into plateau area on the vascular endothelial function and the relationship to acute mountain sickness.

Effect of different blood glucose levels on viscera injury,oxidative stress response and Wnt5a inflammatory pathway in children with sepsis.


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