E11I0308 Human Malondialdehyde ELISA kit
The Human Malondialdehyde ELISA kit can be used to identify samples from the human species. Malondialdehyde can also be called Malondialdehyde MDA.
Specifications of Human Malondialdehyde ELISA kit
Product Information | |
Cat. No. | E01M0023 |
Product Name | Human Malondialdehyde ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 100 ng/mL | 1 vial |
STANDARD C (0.5mL) | 250 ng/mL | 1 vial |
STANDARD D (0.5mL) | 500 ng/mL | 1 vial |
STANDARD E (0.5mL) | 1000 ng/mL | 1 vial |
STANDARD F (0.5mL) | 2500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MDA ELISA kit uses an anti-MDA antibody and an MDA-HRP conjugate in a competitive enzyme immunoassay method. MDA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MDA antibody binding site between MDA from samples and MDA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MDA. Since the number of sites is limited, as more sites are occupied by MDA from the sample, fewer sites are left to bind MDA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MDA concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Malondialdehyde ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MDA and analogues was observed. | |
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Summary of the Assay Procedure for Human Malondialdehyde ELISA kit
Citations of Human Malondialdehyde ELISA kit
E01M0023 has been referenced in the below publications：
The changes of mitochondrial DNA levels in peripheral blood monouclear cells in patients with chronic hepatitis b receiving long- term adefovir treatment.
The Clinical Study on the effect of Kun Fu Kang Capsule on Red Cell Immunology with Chronic Pelvic Inflammatory Disease.
A cell model of nonalcoholic steatohepatitis induced by FFA: establishment and dynamic monitoring.
Entecavir treatment causes injury to the mitochondrial DNA of peripheral blood mononudear cells.
Effects of rapid entrance into plateau area on the vascular endothelial function and the relationship to acute mountain sickness.
Effect of different blood glucose levels on viscera injury,oxidative stress response and Wnt5a inflammatory pathway in children with sepsis.
