E01L0021 Human Luteinizing Hormone ELISA kit
The Human Luteinizing Hormone ELISA kit can be used to identify samples from the human species. Luteinizing Hormone can also be called ICSH, Lutropin, Interstitial Cell Stimulating Hormone.
Specifications of Human Luteinizing Hormone ELISA kit
Product Information | |
Cat. No. | E01L0021 |
Product Name | Human Luteinizing Hormone ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 mIU/mL |
Sensitivity | 1.0 mIU/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mIU/mL | 1 vial |
STANDARD B (0.5mL) | 10 mIU/mL | 1 vial |
STANDARD C (0.5mL) | 25 mIU/mL | 1 vial |
STANDARD D (0.5mL) | 50 mIU/mL | 1 vial |
STANDARD E (0.5mL) | 100 mIU/mL | 1 vial |
STANDARD F (0.5mL) | 250 mIU/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LH ELISA kit uses an anti-LH antibody and an LH-HRP conjugate in a competitive enzyme immunoassay method. LH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LH antibody binding site between LH from samples and LH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LH. Since the number of sites is limited, as more sites are occupied by LH from the sample, fewer sites are left to bind LH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LH concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Luteinizing Hormone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LH and analogues was observed. | |
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Summary of the Assay Procedure for Human Luteinizing Hormone ELISA kit
Citations of Human Luteinizing Hormone ELISA kit
E01L0021 has been not referenced.
