E01C0473 Human Carbohydrate Antigen 125 ELISA kit
The Human Carbohydrate Antigen 125 ELISA kit can be used to identify samples from the human species. Carbohydrate Antigen 125 can also be called MUC16, CA125, mucin 16, cell surface associated.
Specifications of Human Carbohydrate Antigen 125 ELISA kit
Product Information | |
Cat. No. | E01C0473 |
Product Name | Human Carbohydrate Antigen 125 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 U/mL |
Sensitivity | 1.0 U/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 U/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 U/mL | 1 vial |
STANDARD C (0.5mL) | 10 U/mL | 1 vial |
STANDARD D (0.5mL) | 25 U/mL | 1 vial |
STANDARD E (0.5mL) | 50 U/mL | 1 vial |
STANDARD F (0.5mL) | 100 U/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CA125 ELISA kit uses an anti-CA125 antibody and an CA125-HRP conjugate in a competitive enzyme immunoassay method. CA125-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CA125 antibody binding site between CA125 from samples and CA125-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CA125. Since the number of sites is limited, as more sites are occupied by CA125 from the sample, fewer sites are left to bind CA125-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA125 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Carbohydrate Antigen 125 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CA125 and analogues was observed. | |
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Summary of the Assay Procedure for Human Carbohydrate Antigen 125 ELISA kit
Citations of Human Carbohydrate Antigen 125 ELISA kit
E01C0473 has been referenced in the below publications：
The application of TSGF CA125 CEA joint detection in the diagnosis of oophoroma.
