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BlueGene Biotech Human Total Bilirubin ELISA kit (E11I0308)

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E01T0143 Human Total Bilirubin ELISA kit

The Human Total Bilirubin ELISA kit can be used to identify samples from the human species. Total Bilirubin can also be called BR.

Products

Specifications of Human Total Bilirubin ELISA kit

Product Information

Cat. No.

E01T0143

Product Name

Human Total Bilirubin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 nmol/ml

Sensitivity

1.0 nmol/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

nmol/ml

1 vial

STANDARD B (0.5mL)

5.0 nmol/ml

1 vial

STANDARD C (0.5mL)

10 nmol/ml

1 vial

STANDARD D (0.5mL)

25 nmol/ml

1 vial

STANDARD E (0.5mL)

50 nmol/ml

1 vial

STANDARD F (0.5mL)

100 nmol/ml1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TBIL ELISA kit uses an anti-TBIL antibody and an TBIL-HRP conjugate in a competitive enzyme immunoassay method. TBIL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TBIL antibody binding site between TBIL from samples and TBIL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TBIL. Since the number of sites is limited, as more sites are occupied by TBIL from the sample, fewer sites are left to bind TBIL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TBIL concentration in each sample is interpolated from this standard curve.


Quality Control on Human Total Bilirubin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TBIL and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Total Bilirubin ELISA kit

Summary of the Assay Procedure for Human Total Bilirubin ELISA kit

Citations of Human Total Bilirubin ELISA kit

E01T0143 has been referenced in the below publications:

Mild hypothermia inhibits systemic and cerebral complement activation in a swine model of cardiac arrest.

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