E11L0301 Bovine Lysylpyridinoline ELISA kit
The Bovine Lysylpyridinoline ELISA kit can be used to identify samples from the bovine species. Lysylpyridinoline can also be called LP.
Specifications of Bovine Lysylpyridinoline ELISA kit
Product Information | |
Cat. No. | E11L0301 |
Product Name | Bovine Lysylpyridinoline ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ug/mL |
Sensitivity | 0.1 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 nmol/L | 1 vial |
STANDARD B (0.5mL) | 25 nmol/L | 1 vial |
STANDARD C (0.5mL) | 50 nmol/L | 1 vial |
STANDARD D (0.5mL) | 100 nmol/L | 1 vial |
STANDARD E (0.5mL) | 250 nmol/L | 1 vial |
STANDARD F (0.5mL) | 500 nmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LP ELISA kit uses an anti-LP antibody and an LP-HRP conjugate in a competitive enzyme immunoassay method. LP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LP antibody binding site between LP from samples and LP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LP. Since the number of sites is limited, as more sites are occupied by LP from the sample, fewer sites are left to bind LP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LP concentration in each sample is interpolated from this standard curve. |
Quality Control on Bovine Lysylpyridinoline ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LP and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Bovine Lysylpyridinoline ELISA kit
Citations of Bovine Lysylpyridinoline ELISA kit
E11L0301 has been referenced in the below publications：
Study on the Relationships Between Pyridinoline Cross-Links and Solubility of Collagen and Shear Force of Qinchuan Marbling Beef.
EFFECTS OF AGE AND FAT DEPOSITION ON BEEF TENDERNESS.
