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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Melatonin ELISA kit (E11M0005)

E11M0005 Bovine Melatonin ELISA kit

The Bovine Melatonin ELISA kit can be used to identify samples from the bovine species. Melatonin can also be called MLT.

Products

Specifications of Bovine Melatonin ELISA kit

Product Information

Cat. No.

E11M0005

Product Name

Bovine Melatonin ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MLT ELISA kit uses an anti-MLT antibody and an MLT-HRP conjugate in a competitive enzyme immunoassay method. MLT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MLT antibody binding site between MLT from samples and MLT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MLT. Since the number of sites is limited, as more sites are occupied by MLT from the sample, fewer sites are left to bind MLT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MLT concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Melatonin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MLT and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Melatonin ELISA kit

Summary of the Assay Procedure for Bovine Melatonin ELISA kit

Citations of Bovine Melatonin ELISA kit

E11M0005 has been referenced in the below publications:

Effect of exposure to extremely low frequency magnetic fields on melatonin levels in calves is seasonally dependent.

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