E11N0063 Bovine Non ester Fatty Acid ELISA kit
The Bovine Non ester Fatty Acid ELISA kit can be used to identify samples from the bovine species. Non ester Fatty Acid can also be called FFA, free fatty acid, NEFA.
Specifications of Bovine Non ester Fatty Acid ELISA kit
Product Information | |
Cat. No. | E11N0063 |
Product Name | Bovine Non ester Fatty Acid ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 µmol/L |
Sensitivity | 0.1 µmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 µmol/L | 1 vial |
STANDARD B (0.5mL) | 0.5 µmol/L | 1 vial |
STANDARD C (0.5mL) | 1.0 µmol/L | 1 vial |
STANDARD D (0.5mL) | 2.5 µmol/L | 1 vial |
STANDARD E (0.5mL) | 5.0 µmol/L | 1 vial |
STANDARD F (0.5mL) | 10 µmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
NEFA ELISA kit uses an anti-NEFA antibody and an NEFA-HRP conjugate in a competitive enzyme immunoassay method. NEFA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NEFA antibody binding site between NEFA from samples and NEFA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NEFA. Since the number of sites is limited, as more sites are occupied by NEFA from the sample, fewer sites are left to bind NEFA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NEFA concentration in each sample is interpolated from this standard curve. |
Quality Control on Bovine Non ester Fatty Acid ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between NEFA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Bovine Non ester Fatty Acid ELISA kit
Citations of Bovine Non ester Fatty Acid ELISA kit
E11N0063 has been referenced in the below publications：
Effect of Different Feeding Patterns on Serum Biochemical Indices and Hormone Content in Dairy Cows.
