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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Progesterone ELISA kit (E11P0200)

E11P0200 Bovine Progesterone ELISA kit

The Bovine Progesterone ELISA kit can be used to identify samples from the bovine species. Progesterone can also be called P4, Pregn-4-Ene-3,20-Dione, PROG.

Products

Specifications of Bovine Progesterone ELISA kit

Product Information

Cat. No.

E11P0200

Product Name

Bovine Progesterone ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PROG ELISA kit uses an anti-PROG antibody and an PROG-HRP conjugate in a competitive enzyme immunoassay method. PROG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PROG antibody binding site between PROG from samples and PROG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PROG. Since the number of sites is limited, as more sites are occupied by PROG from the sample, fewer sites are left to bind PROG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PROG concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Progesterone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PROG and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Progesterone ELISA kit

Summary of the Assay Procedure for Bovine Progesterone ELISA kit

Citations of Bovine Progesterone ELISA kit

E11P0200 has been referenced in the below publications:

impact of fertility-promoting powder on trace elements and hormone in serum of dairy cattle with ovarian static disease.

A Study on the Impact of Reproductive Hormones in Serum by Using Fertility-Promoting Powder for Treating Infertility Caused by Persistent Corpus Luteum and Ovary Sandstill of Dairy Cattle.

study on the impact of fertility-promoting powder on reproductive hormones in serum of dairy cattle with persistent corpus luteum.

Study on Effect of Reproductive Performance of Cows on Biotin and Endocrine Mechanism.

Study on the Dagnosis Ovarian Disease by B-mode Ultrasound Scanner and Optimization of the Treatment Protocol on Different Stage of Postpartum in Dairy Cows.

奶牛排卵延迟的综合诊断。

中药复方理囊散对奶牛子宫复旧期间生殖激素的影响。

The correlation analysis of trace elements and hormones in serum of infertile dairy cattle with ovarian stationary treated with fertility-promoting powder.

Correlation Analysis of IL and Hormone in Serum During Treatment of Persistent Courpus Luteum with Fertility-promoting Power in Dairy Cows.

Rule Analysis of LH,E2 and P4 of Ovulation-Delayed Dairy Cows at Different Stages after Estrus.

奶牛卵巢囊肿的综合诊断。

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