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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Prostaglandin E2 ELISA kit (E11P0012)

E11P0012 Bovine Prostaglandin E2 ELISA kit

The Bovine Prostaglandin E2 ELISA kit can be used to identify samples from the bovine species. Prostaglandin E2 can also be called EP 2, EP2, Prostaglandin E Receptor EP2, PGE receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostaglandin E receptor 2 EP2 subtype, Prostaglandin E receptor 2 subtype EP2 53kDa, Prostaglandin E receptor 2 subtype EP2, Prostaglandin E2 receptor, Prostaglandin E2 receptor EP2 subtype, Prostanoid EP2 receptor, PTGER 2, PTGER2, PGE2.

Products

Specifications of Bovine Prostaglandin E2 ELISA kit

Product Information

Cat. No.

E11P0012

Product Name

Bovine Prostaglandin E2 ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PGE2 ELISA kit uses an anti-PGE2 antibody and an PGE2-HRP conjugate in a competitive enzyme immunoassay method. PGE2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PGE2 antibody binding site between PGE2 from samples and PGE2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PGE2. Since the number of sites is limited, as more sites are occupied by PGE2 from the sample, fewer sites are left to bind PGE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGE2 concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Prostaglandin E2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PGE2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Prostaglandin E2 ELISA kit

Summary of the Assay Procedure for Bovine Prostaglandin E2 ELISA kit

Citations of Bovine Prostaglandin E2 ELISA kit

E11P0012 has been referenced in the below publications:

Changes of hematological and biochemical parameters and levels of pepsinogen, histamine and prostaglandins in dairy cows affected with left displacement of the abomasum.

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