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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Prostaglandin F2a ELISA kit (E11P0652)

E11P0652 Bovine Prostaglandin F2a ELISA kit

The Bovine Prostaglandin F2a ELISA kit can be used to identify samples from the bovine species. Prostaglandin F2a can also be called PGF2A, PGF2-A, PG-F2a.

Products

Specifications of Bovine Prostaglandin F2a ELISA kit

Product Information

Cat. No.

E11P0652

Product Name

Bovine Prostaglandin F2a ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PGF2a ELISA kit uses an anti-PGF2a antibody and an PGF2a-HRP conjugate in a competitive enzyme immunoassay method. PGF2a-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PGF2a antibody binding site between PGF2a from samples and PGF2a-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PGF2a. Since the number of sites is limited, as more sites are occupied by PGF2a from the sample, fewer sites are left to bind PGF2a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGF2a concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Prostaglandin F2a ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PGF2a and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Prostaglandin F2a ELISA kit

Summary of the Assay Procedure for Bovine Prostaglandin F2a ELISA kit

Citations of Bovine Prostaglandin F2a ELISA kit

E11P0652 has been referenced in the below publications:

Changes of hematological and biochemical parameters and levels of pepsinogen, histamine and prostaglandins in dairy cows affected with left displacement of the abomasum.

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