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BlueGene Biotech Bovine Pulmonary Surfactant Associated Protein B ELISA kit (E11S0163)

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E11S0163 Bovine Pulmonary Surfactant Associated Protein B ELISA kit 

The Bovine Pulmonary Surfactant Associated Protein B ELISA kit can be used to identify samples from the bovine species. Pulmonary Surfactant Associated Protein B can also be called SPB, SFTPB, PSP-B, SFTB3, SFTP3, SMDP1, SP-B, surfactant protein B, Surfactant protein B.

Products

Specifications of Bovine Pulmonary Surfactant Associated Protein B ELISA kit

Product Information

Cat. No.

E11S0163

Product Name

Bovine Pulmonary Surfactant Associated Protein B ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/ml

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SPB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SPB antibody and an SPB-HRP conjugate. The assay sample and buffer are incubated together with SPB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SPB concentration since SPB from samples and SPB-HRP conjugate compete for the anti-SPB antibody binding site. Since the number of sites is limited, as more sites are occupied by SPB from the sample, fewer sites are left to bind SPB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPB concentration in each sample is interpolated from this standard curve. 


Quality Control on Bovine Pulmonary Surfactant Associated Protein B ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SPB and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Pulmonary Surfactant Associated Protein B ELISA kit

Summary of the Assay Procedure for Bovine Pulmonary Surfactant Associated Protein B ELISA kit

Citations of Bovine Pulmonary Surfactant Associated Protein B ELISA kit

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