Bovine Pulmonary Surfactant Associated Protein B ELISA kit (E11S0163)

BlueGene Biotech Bovine Pulmonary Surfactant Associated Protein B ELISA kit (E11S0163)

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E11S0163 Bovine Pulmonary Surfactant Associated Protein B ELISA kit

The Bovine Pulmonary Surfactant Associated Protein B ELISA kit can be used to identify samples from the bovine species. Pulmonary Surfactant Associated Protein B can also be called SPB, SFTPB, PSP-B, SFTB3, SFTP3, SMDP1, SP-B, surfactant protein B, Surfactant protein B.

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Specifications of Bovine Pulmonary Surfactant Associated Protein B ELISA kit

Product Information
Cat. No.	E11S0163
Product Name	Bovine Pulmonary Surfactant Associated Protein B ELISA kit
Species	Bovine
Product Size	48 Tests / 96 Tests
Concentration	25-500 ng/ml
Sensitivity	1.0 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	25 ng/mL	1 vial
STANDARD C (0.5mL)	50 ng/mL	1 vial
STANDARD D (0.5mL)	100 ng/mL	1 vial
STANDARD E (0.5mL)	250 ng/mL	1 vial
STANDARD F (0.5mL)	500 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

SPB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SPB antibody and an SPB-HRP conjugate. The assay sample and buffer are incubated together with SPB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SPB concentration since SPB from samples and SPB-HRP conjugate compete for the anti-SPB antibody binding site. Since the number of sites is limited, as more sites are occupied by SPB from the sample, fewer sites are left to bind SPB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPB concentration in each sample is interpolated from this standard curve.

Quality Control on Bovine Pulmonary Surfactant Associated Protein B ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between SPB and analogues was observed.