E11G0367 Bovine Reduced glutathione ELISA kit
The Bovine Reduced glutathione ELISA kit can be used to identify samples from the bovine species. Reduced glutathione can also be called L-Reduced Glutathione, GSH.
Specifications of Bovine Reduced glutathione ELISA kit
Product Information | |
Cat. No. | E11G0367 |
Product Name | Bovine Reduced glutathione ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GSH ELISA kit uses an anti-GSH antibody and an GSH-HRP conjugate in a competitive enzyme immunoassay method. GSH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GSH antibody binding site between GSH from samples and GSH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GSH. Since the number of sites is limited, as more sites are occupied by GSH from the sample, fewer sites are left to bind GSH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSH concentration in each sample is interpolated from this standard curve. The Bovine Cortisol ELISA Kit utilizes a competitive enzyme immunoassay method with an anti-cortisol antibody and a cortisol-HRP conjugate to measure cortisol levels in bovine samples, with the color intensity inversely proportional to the cortisol concentration. These can be provided by bluegene biotech. |
Quality Control on Bovine Reduced glutathione ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GSH and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Bovine Reduced glutathione ELISA kit
Citations of Bovine Reduced glutathione ELISA kit
E11G0367 has been referenced in the below publications：
Low Expression of Sirtuin 1 in the Dairy Bovines with Mild Fatty Liver Alters Hepatic Lipid Metabolism.
