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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Serum amyloid A ELISA kit (E11S0228)

E11S0228 Bovine Serum amyloid A ELISA kit

The Bovine Serum amyloid A ELISA kit can be used to identify samples from the bovine species. Serum amyloid A can also be called amyloid A, serum, Amyloid fibril protein AA, Amyloid protein A, PIG4, SAA, SAA2, Serum amyloid A protein, serum amyloid A-1 protein, serum amyloid A1, TP53I4, Tumor protein p53 inducible protein 4.

Products

Specifications of Bovine Serum amyloid A ELISA kit

Product Information

Cat. No.

E11S0228

Product Name

Bovine Serum amyloid A ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SAA ELISA kit uses an anti-SAA antibody and an SAA-HRP conjugate in a competitive enzyme immunoassay method. SAA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SAA antibody binding site between SAA from samples and SAA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SAA. Since the number of sites is limited, as more sites are occupied by SAA from the sample, fewer sites are left to bind SAA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SAA concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Serum amyloid A ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SAA and analogues was observed.


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Summary of the Assay Procedure for Bovine Serum amyloid A ELISA kit

Summary of the Assay Procedure for Bovine Serum amyloid A ELISA kit

Citations of Bovine Serum amyloid A ELISA kit

E11S0228 has been referenced in the below publications:

Effect of non-esterified fatty acid and beta-hydroxybutyrate on the haptoglobin and serum amyloid A protein of bovine hepatocyte in vitro.

Comparative study of haptoglobin and serum amyloid A protein in the serum of healthy dairy cows and cows with ketosis.

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